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Biogeochemical cycling of phosphorus: Insights from oxygen isotope effects of phosphoenzymes

Ruth E. Blake^*,, James R. O’Neil^** and Aleksandr V. Surkov^*

^* Department of Geology and Geophysics, Yale University, P.O. Box 208109, New Haven, Connecticut 06520-8109
^** Department of Geological Sciences, University of Michigan, Ann Arbor, Michigan 48109

Corresponding author: ruth.blake{at}yale.edu

Geochemical cycling of phosphorus (P) in aquatic environments is carried out almost exclusively by biota and involves reactions that are catalyzed by enzymes. Oxygen isotope effects accompanying phosphoenzymatic reactions have been determined in controlled laboratory experiments in order to elucidate processes underlying biogeochemical cycling of P, and to identify possible reaction pathways for P-compounds in nature. Phosphate oxygen isotope effects are distinct for specific enzymatic reaction mechanisms measured in microbial culture experiments and in cell-free systems. P¹⁶O₄ is taken up preferentially from inorganic phosphate (P_i) in the growth medium by intact E. coli cells, producing a kinetic fractionation in the extracellular dissolved P_i pool. Inorganic pyrophosphatase is the intracellular enzyme that catalyzes the temperature-dependent equilibrium oxygen isotope fractionations between phosphates and water in biological systems, and imprints an equilibrium isotope signature on P_i that is turned over or cycled by intact cells. Alkaline phosphatase, a key enzyme involved in extracellular P_i regeneration in aquatic systems, catalyzes hydrolysis of phosphomonoesters, reactions that are accompanied by kinetic fractionations and disequilibrium (inheritance) isotope effects in released P_i. Comparison of laboratory determined enzyme-specific isotopic fractionations with those observed in microbial culture experiments and in natural aquatic systems, provide new insights into processes controlling P cycling and the relations between P availability and the cycling of N and C. Isotopic signatures associated with specific cellular processes and phosphoenzyme reaction pathways may be useful in assessing P status and for tracing P turnover.

Key Words: P—phosphorus • P_i—dissolved inorganic orthophosphate (PO₄) • P_org—organophosphate, any organic compound containing PO₄ moieties or P-O bonds • PP_i—inorganic pyrophosphate • PPase—inorganic pyrophosphatase • APase—alkaline phosphatase • ¹⁸O_P—oxygen isotope composition of P_i in permil relative to SMOW • ¹⁸O_W—oxygen isotope composition of water in permil relative to SMOW

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